Results showed that 78% of shEGFP +ve cells were positive for excitatory neurons which contain Vglut2 (vesicular glutamate transporter) whereas 22% were positive for inhibitory neurons which contain GAD65/67 (glutamate decarboxylase 65/67) (Figures 1G,H, in Wang et al., 2022). The shEGFP+ neurons were individually isolated and subjected to single-cell RT-PCR to determine the co-expression of a number of other neuronal markers. shEGFP +ve and c-Fos +ve neurons were found mainly in the superficial layer of the dorsal horn of the spinal cord where nociceptive primary afferents (mainly C and Aδ fibers) normally terminate. The authors confirmed that those shEGFP +ve neurons also overlapped with the same neurons labeled with c-Fos antibody, demonstrating its validity as a good indicator of activated neurons in the dorsal horn of the spinal cord. ![]() ![]() ![]() When mice were exposed to noxious heat stimuli by using 52☌ hot plate, compared with mice exposed to 25☌ plate, shEGFP expression was increased in neurons of spinal cord dorsal horns (Figures 1B,C, in Wang et al., 2022). In this study the authors used c-Fos::shEGFP mice that express a short half-life enhanced green fluorescent protein (shEGFP) under the control of endogenous c-Fos promoter to characterize the neurons which are involved in heat sensations. ![]() We have read with great interest the manuscript entitled “A novel spinal neuron connection for heat sensation” by Wang et al.
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